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Table 2 Identification of nucleolin associated proteins in the 500-kDa complex.

From: Targeting surface nucleolin with multivalent HB-19 and related Nucant pseudopeptides results in distinct inhibitory mechanisms depending on the malignant tumor cell type

Protein Band (MW)

Obtained Sequence 1 /Medline Sequence 2

Band 5 (90-kDa)

 MHRPFXX 1

 

1-MHRNFRK 2 = Wnt related protein A 3

Band 6 (85-kDa)

 MRPMTQIIVQD 1

 

1-MRPMTFIVGLK 2 = Wnt related protein B 4

Band 7 (80-kDa)

 VRSGNKAAVVLXMDVXFTMS 1

 

1-MVRSGNKAAVVLCMDVGFTMS = Ku80 5

Band 8 (72-kDa)

 ASGGSGGVXVPA 1

 

1-MASGGSGGVSVPA 2 = SRP72 6

Band 9 (68-kDa)

 AAEKQVPGGXXGGGS 1

 

1-MAAEKQVPGGGGGGGS 2 = SRP68 6

Band 11 (32-kDa)

 LHTDGDKAFVDFLSD 1

 

74-LHTDGDKAFVDFLSD 2 = p32/p33/HABP1/gC1q-R 7

Bands 12 (25-kDa):

 ARGPKKHLK 1

 

1-MARGPKKHLK 2 = S4 8

 

 KLNISFPAL 1

 

1-MKLNISFPAL 2 = S6 8

  1. By the capacity of the HB-19/Btn to bind surface nucleolin, the 500-kDa complex was recovered from the cell surface and purified using avidin-agarose. After analysis by SDS-PAGE, several protein bands of 90-, 85-, 80-, 72-, 68-, 32, and 25-kDa were processed for NH2-terminal microsequencing (Methods; Additioal file 2, Figure 2S). These protein bands were identified as Wnt related proteins, Ku80, SRP68, SRP72, p32/p33/HABP1/gC1q-R, and ribosomal proteins S4 and S6.
  2. 1 The obtained NH2-terminal sequence.
  3. 2 The NH2-terminal sequence in the Medline.
  4. 3 The obtained MHRPFXX sequence shares homology to the N-terminal sequence of human Wnt-7b (MHRNFR) [87].
  5. 4 The obtained MRPMTQIIVQD sequence is homologous in its first 5 amino acid residues with the N-terminal sequence (MRPMTFIVGLK) of Wnt-1 of the Mexican axolotls Ambystoma mexicanum [88]. Consequently, the isolated protein appears to correspond to a human Wnt-related protein that remains to be described.
  6. 5 The 80-kDa subunit of the Ku autoantigen [89]
  7. 6 The 68- and 72-kDa subunit of the signal recognition particle [60].
  8. 7 Referred in the literature as the protein p32 associated with the splicing factor SF2, the hyaluronan binding protein 1 (HABP1) and the receptor for complement component C1q (gC1q-R)[90].
  9. 8 Ribosomal proteins S4 and S6 [91].
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BMC Cancer

ISSN: 1471-2407

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