Figure 4
The inhibitory effect of Nucant on cell adhesion occurs without any apparent cytotoxicity. A. Inhibition of Cell adhesion by N6. MDA-MB 231, MDA-MB-435 and LNCaP (1 × 106) cells were passaged in 25 cm3 flasks in the absence (lanes 0 μM for control) or presence of 5, 10, and 20 μM of N6. After 16 hours of culture, adherent cells were trypsinized and the number of cells in each flask was monitored in the presence of trypan blue. B. Cell multiplication is not affected in Nucant cultured cells. Cells were seeded at 2 × 105 cells in 25 cm3 flasks. When cells reached subconfluency (after 48 hours), they were further cultured for 24 hours in the presence of different concentrations of N6 before counting. C. The effect of Nucant treatment on cell proliferation. Seven hours after seeding (1 × 105 cells in 25 cm3 flasks), cells were cultured in the presence of different concentrations of N6 and counted after four days. The ordinates give the mean ± SD of triplicate samples. Statistical significance: **p < 0.01, ***p < 0.001.