Cell proliferation, apoptosis and Na+-K+ ATPase expression in lung cancer tumors. Immunohistochemistry was used to evaluate cell proliferation and apoptosis. Mitosis was analyzed in slides with H & E stain. (A) Percent Ki67 positive cell number for cell proliferation; (B) Mitosis index and (C) Apoptosis. Left panel showing quantitative data and right panel showing representative micrographs. n = 5 for each group. Expression of Na
ATPase α1 in lung cancer tumors and colon cancer tumors (D to F): Proteins were isolated from the tumors and Western blot was performed for analysis of Na+-K+ ATPase α1 expression. Expression of Na+-K+ ATPase α1 in lung cancer tumor in rats (D) and in A549 cells and in the lung cancer tumor from hypoxia-pretreated A549 cells in mice (E). (F) Na+-K+ ATPase α1 expression in colon cancer tumor in rats (F). n = 3 for each group. Upper panels show representative images and lower panels show quantitative data, setting normoxia as 1. *p < 0.05 as compared with normoxia.