Figure 2

The expression and different subcellular localization of CD133 in CC. Among the CD133-positive cholangiocarcinoma, three staining patterns were identified: the well-differentiated tumors (A) were stained mostly on the apical membrane (D) or both membrane and cytoplasm (E) but the majority of moderately (B) and poorly differentiated (C) tumors were stained in the cytoplasm (F) or both membrane and cytoplasm, original magnification 400×. Also, in some cases, CD133-positive cellular debris was observed in the malignant glandular lumina (E).