Figure 4From: Tumor-suppressor activity of RRIG1 in breast cancer RRIG1 regulation of gene expression and activities. All the experiments were repeated at least once with similar results. A, Western blotting. The RRIG1 sense and antisense-transfected MDA-MB-231 and MDA-MB-435 cells, respectively, were grown in G418-containing medium for 5 days and then subjected to protein extraction and western blotting analysis of gene expression. The value shown is the percentage of control, which was calculated using the formula: value of the RRIG1 sense or antisense transfected cells/value of the vector-only transfected cells after intensity of the target band of proteins from western blots was quantified by using NIH ImageJ 1.34s software and normalized to β-actin. B, Semiquantitative RT-PCR. The gene-transfected cells were grown in G418-containing medium for 5 days, and RNA was then isolated from the cells and subjected to semiquantitative RT-PCR analysis of MMP9 expression. C, RhoA activation assay. The gene-transfected cells were grown in G418-containing medium for 5 days and then subjected to the RhoA activation and western blot assays. The data were quantified using the NIH ImageJ software.Back to article page