Figure 3

Tumor cell migration and invasion assays. All experiments were performed in triplicate and repeated twice. A, Migration assay. The RRIG1 sense and antisense-transfected MDA-MB-231 and MDA-MB-435 cells, respectively, were grown in G418-containing medium and then subjected to cell migration assay in Boyden chambers without Matrigel for 24 h. The cells that migrated were stained with 1% crystal violet solution and then counted and summarized. B, Invasion assay. These gene-transfected breast cancer cells were grown in G418-containing medium and then subjected to the cell invasion assay in Boyden chambers with Matrigel for 48 h. The invaded cells were stained with 1% crystal violet solution and then counted and summarized. *p < 0.05 vs. the control.