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Table 2 Growth curves in glioma cells exposed to vehicle or ketone bodies

From: Differential utilization of ketone bodies by neurons and glioma cell lines: a rationale for ketogenic diet as experimental glioma therapy

21% O2 3OHB AcAc LiCl 3OHB + AcAc 3OHB + LiCl
U87MG 94.9 ± 9.9 109.7 ± 10.3 104.5 ± 11.7 107.3 ± 10.1 100.8 ± 10.0
U251MG 100.2 ± 5.8 97.4 ± 5.5 97.0 ± 6.9 96.1 ± 5.4 90.2 ± 5.0
LNT-229 98.4 ± 4.7 100.6 ± 3.7 109.5 ± 7.9 104.0 ± 3.6 95.7 ± 4.0
T98G 96.3 ± 4.8 87.6 ± 4.0 91.2 ± 7.8 86.1 ± 5.3 88.0 ± 4.1
A172 97.1 ± 6.0 100.3 ± 5.7 99.9 ± 7.4 84.9 ± 10.7 90.7 ± 5.6
LNT-229 scrambled sh 99.9 ± 9.5 109.2 ± 6.0 103.7 ± 5.3 102.2 ± 6.2 97.5 ± 6.8
LNT-229 p53sh 98.3 ± 5.3 102.3 ± 3.2 96.5 ± 2.4 95.1 ± 2.4 95.0 ± 2.8
0.1% O 2 3OHB AcAc LiCl 3OHB + AcAc 3OHB + LiCl
U87MG 97.7 ± 4.9 115.8 ± 5.8 115.2 ± 4.9 108.8 ± 4.2 104.0 ± 5.7
U251MG 96.6 ± 2.7 102.6 ± 5.0 98.1 ± 2.5 92.1 ± 5.4 86.9 ± 3.0
LNT-229 93.6 ± 3.5 103.2 ± 4.9 104.4 ± 3.3 97.0 ± 3.3 91.0 ± 5.1
T98G 98.8 ± 10.1 100.2 ± 9.1 97.3 ± 8.7 101.7 ± 7.3 91.5 ± 9.9
A172 107.9 ± 15.6 93.2 ± 9.2 95.8 ± 7.4 101.0 ± 8.7 91.6 ± 6.8
LNT-229 scrambled sh 99.6 ± 5.4 104.5 ± 4.7 105.4 ± 6.0 98.9 ± 4.6 103.9 ± 8.2
LNT-229 p53sh 94.8 ± 2.5 107.4 ± 4.2 107.6 ± 5.4 102.9 ± 4.0 96.0 ± 4.5
  1. Cells were exposed to 3-hydroxybutyrate (3OHB, 5 mM), acetoacetate (AcAc, 5 mM), lithium chloride (LiCl, 5 mM) or both for 36 h at 21% or 0.1% O2 and subsequently stained with crystal violet. Data are expressed as percentages (mean and standard deviation) relative to untreated control cells.