Figure 2

HRMA of MUTYH exon 13. All heterozygous and homozygous variants in exon 13 are clearly distinguishable from each other and from wild-type samples without mixing with reference DNA. A) Normalized melting curves obtained for samples carrying variants c.1187G > A (both homozygous and heterozygous), c.1187-27C > T + c.1249C > A (double heterozygous), and wild-type samples; B) Normalized melting curves obtained for c.1187G > A (both homozygous and heterozygous) and homozygous c.1227_1228dupGG, and wild-type samples. Homo: homozygote; het: heterozygote; WT: wild-type; deg: degrees (°C).