Figure 1

HRMA of MUTYH exon 12. The homozygous c.1014G > C transversion is visible with HRMA only when samples are mixed with wild-type DNA. A) HRMA of native test samples: the normalized melting curves of c.1014G > C heterozygotes are distinct from those of GG wild-type homozygotes; the melting profiles of GG and CC homozygotes are clustered together. B) After mixing with a reference DNA, the CC sample is clearly distinguishable from both heterozygous GC and homozygous GG samples. Homo: homozygote; het: heterozygote; WT: wild-type; deg: degrees (°C).