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Figure 3 | BMC Cancer

Figure 3

From: Glioblastoma-derived Leptin Induces Tube Formation and Growth of Endothelial Cells: Comparison with VEGF Effects

Figure 3

Leptin and VEGF enhance angiogenesis and growth of HUVEC. ObR and VEGFR inhibitors counteract these effects. A) Representative phase contrast images (magnification 10×) of ES formed by HUVEC treated with 100 ng/mL leptin and/or with Aca1 or with 50 ng/mL VEGF and/or with SU1498 or left untreated (control) for 8 h. B) HUVEC growing in VCBM were treated for 24 h with 200 ng/mL leptin and/or Aca1 10, 25 or 50 nM or with 50 ng/mL VEGF and/or or with SU1498 1 and 5 μM or left untreated (control). The graph represents relative growth increase with untreated cells taken as reference (0%). Columns, mean of at least three independent experiments done in triplicates; bars, SE. * P < 0,05 and ** P < 0,01 (Leptin or VEGF vs. untreated cells, leptin + Aca1 vs. leptin or VEGF + SU1498 vs. VEGF). C) HUVEC were pretreated for 1 h with ObR or VEGFR inhibitors and then treated with 200 ng/mL leptin or 50 ng/mL VEGF for 15 min or left untreated as described in Materials and Methods. Levels of pSTAT3 and total STAT3 (~88 kDa) were determined by WB in 50-70 μg of total cell lysates using specific Abs, as described in Material and Methods. Arrows indicate the position of 105 kDa molecular weight marker.

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