Effect of transient ectopic RUNX3 expression. CAT (control) and RUNX3 constructs were introduced into HCC cell lines. After a 48-h incubation period, an immunoblot analysis for RUNX3 expression (A), a DAPI apoptosis detection assay (B), and an immunoblot analysis for Bim and caspase (C) were performed. Shown here are representative blots from more than three independent experiments. All results are expressed as ratios to control CAT-transfected cells (CAT; white bars, RUNX3; black bars). Data represent the mean ± S.E. of more than five independent experiments, each with triplicates. **, P < 0.01 (vs. data at 0 h); Student's t-test.