Effect of Bim siRNA gene silencing on cell growth inhibition and apoptosis. After treatment with Bim siRNA duplexes, an immunoblot analysis (A) and DAPI apoptosis detection assay (B) were performed. Shown here are representative blots from more than three independent experiments. All results are expressed as ratios to control siRNA-treated cells (control siRNA, white bars; Bim siRNA, black bars). Data are mean ± S.E. from at least three independent experiments, each performed in triplicate. Statistical analysis was performed with the Student's t-test. n.s., not significant; **, P < 0.01 (vs. control siRNA-treated cells).