Figure 2

NDRG2 modulates CD24 expression in HCC cells. NDRG2-low HCC MHCC97H cells were infected with adenovirus expressing NDRG2 (Ad-NDRG2) or the negative control gene Lac Z (Ad-LacZ) (A-C), while NDRG2-high HCC Huh7 cells were transfected with NDRG2 siRNA (siRNA-N2) or negative control siRNA (siRNA-NC) using Lipofectamine 2000 (D-F). RNA and protein were extracted and subjected to qRT-PCR (A, B, D and E) and western blotting (C and F), respectively. β-actin was used as a loading control. Data represent three independent experiments. *P < 0.05 between groups.