In vitro protein expression of OPN, thrombin, TF, and OPN-related receptors in 468-CON, 468-OPN and 468-ΔTC cells. (A)Protein expression of OPN, thrombin, and tissue factor (TF) was analyzed by western blot analysis of conditioned media from 5 × 105 cultured cells for each cell line as described in the Materials and Methods. (B)Receptor expression was measured by flow cytometry analysis. Cultured cells were incubated with specific antibodies (filled profiles) or with a nonspecific isotype control primary antibody (open profiles). Filled profiles represent expression of α9β1 integrin, β1 integrin, β3 integrin, αvβ5 integrin and CD44. Representative results for 468-CON cells only are shown; equivalent expression was observed in all cell lines (data not shown).