Figure 2

Human pancreatic normal stellate cells and adenocarcinoma-derived ECMs resemble FAP ^- and FAP ⁺ ECMs, respectively. Human normal pancreatic stellate cells and pancreatic tumor-associated fibroblasts were isolated from fresh surgical tissue samples corresponding to matched (from the same case) normal pancreas (at least 2 cm away from the tumor) and pancreatic adenocarcinoma. Similar to images shown in Figure 1B, un-extracted human fibroblastic pancreas matrices (anti-fibronectin in green) were subjected to indirect immunofluorescence while cells' nuclei were labeled with DAPI (blue). Note how human pancreatic adenocarcinoma-associated fibroblasts produced ECMs oriented in parallel patterns, which are similar to the patterns formed by FAP⁺ matrices while normal pancreatic stellate cells-derived matrices resemble FAP^- ECMs.