Figure 2

Activation of intrinsic and extrinsic caspase cascades following JAK2 inhibition in JAK2 ^V617F mutant cells lines. SET-2 (A) and MB-02 (B) cells were treated with 500 nM of the JAK2 inhibitor NVP-BSK805 and extracted at the indicated time points for Western blot analysis. Control (Ctrl) cells were treated with the drug vehicle DMSO for 72 hours. Induction of apoptosis markers becomes evident starting at the 16 hours time point in both cell lines. Arrowhead denotes cleaved PARP, running below the band for full-length PARP. The caspase-8 blot shows the p43/p41 cleavage intermediates and active caspase-8 fragment p18.