Figure 3
From: ER stress drives Lipocalin 2 upregulation in prostate cancer cells in an NF-κB-dependent manner
UPR-mediated Lcn2 transcription is NF-κB dependent. (A) TC1.pNGL cells were treated for 8 h with Tg (300 nM) with or without Bay 11-7082 (25 μM), or vehicle alone, and analyzed for EGFP reporter fluorescence by flow cytometry. Cells viability in all treatment groups was > 99%. Results shown are representative of 3 experiments. (MFI Veh: 50; MFI Bay + Tg: 61.8; MFI Tg: 72.7) (B) RNA was isolated from cells from (A) and analyzed for UPR activation and Lcn2, Il-6, and Il-23p19 transcription by RT-qPCR. Data columns indicate the fold difference in transcript level between drug- and vehicle-treated TC1.pNGL cells. Error bars represent SEM of 2-3 biological replicates. Statistical analysis was performed using an unpaired two-tailed t test (**p < 0.01; ***p < 0.001).