Figure 1

Lcn2 and pro-inflammatory cytokine gene transcription in murine prostate cancer cells follows the kinetics of the UPR. (A) TC1 cells were treated with Tg (300 nM) for the indicated times, after which mRNA was isolated and analyzed by RT-qPCR for markers of UPR activation and Lcn2 transcription. Data columns indicate the relative quantification (RQ), or fold difference, in transcript level between Tg- and vehicle-treated TC1 cells. A representative experiment of at least three independent experiments per time point is shown. (B) TC1 cells were cultured in RPMI lacking glucose for 24 h and analyzed by RT-qPCR for markers of UPR activation and Lcn2 transcription. Error bars represent SEM of 2 biological replicates representative of at least 3 independent experiments. Statistical analysis was performed using an unpaired two-tailed t test (**p < 0.01; ***p < 0.001).