Figure 6

Effects of LGD1069 on metalloproteinases and vegf mRNA expression in HUVECs by RT-PCR assay. HUVECs were incubated for 12 h in medium containing vehicle(DMSO, Line 1) or 7.5 μM (Line 2), 15 μM (Line 3), and 30 μM (Line 4) LGD1069, in the presence of 2 ng/ml VEGF. The expression of a housekeeping gene β-actin was used to normalize gene expression level. Error bars represent the standard deviation of three replicate experiments. Panel A: RT-PCR analysis of metalloproteinases and vegf genes expression. Line M was DNA marker. Pane B: densitometric analysis of electrophoretic bands. Intensities were normalized to a β-actin signal. Data were expressed as mean ± S.D. a p < 0.05, b p < 0.01 compared with control.