Figure 4

LGD1069 inhibited the migration and adhesion of HUVECs. For migration assay, HUVECs were seeded in upper compartments of AP48 chamber, and untreated or treated with LGD1069, in the presence of 2 ng/ml VEGF. Cells on the lower face of the membrane were counted by the method described under materials and methods. Vehicle (DMSO) was utilized as a control. b p < 0.01 compared with control (n = 3). For adhesion assay, HUVECs were seeded in Matrigel-coated 96-well plates, and untreated or treated with LGD1069, in the presence of 2 ng/ml VEGF. The number of adhesive cells was evaluated by MTT assay. Vehicle (DMSO) was utilized as a control. Values were normalized to the mean number of adhesive control cells and presented as mean ± SD percentage of adhesion (n = 3). b p < 0.01 compared with control. Panel A is results from migration assay, and panel B shows the results of adhesion assay.