Figure 3

LGD1069 inhibited the invasion of HUVECs in AP48 chamber. HUVECs were seeded in upper compartments of AP48 chamber, and untreated or treated with LGD1069, in the presence of 2 ng/ml VEGF. Cells on the lower face of the membrane were counted by the method described under materials and methods. Vehicle (DMSO) was utilized as a control. Five fields were counted for each chamber, and each concentration repeated three chambers (100X). A. Control; B. LGD1069 7.5 μM; C. LGD1069 15 μM; D. LGD1069 30 μM; E. statistical results. b p < 0.01 compared with control (n = 3).