Cord blood mononuclear cells inhibit tumor growth in human cervical tumor animal model. A, representative tumor formation at 8 weeks in NOD/SCID mice injected subcutaneously with Caski cells (2 × 106 cells) was shown. B, Caski cells (2 × 106 cells) were injected subcutaneously into the posterior region of NOD/SCID mice with 1 × 107 human cord blood mononuclear cells on day 0 or day 14 after the tumor injection. Data are presented on tumor size and weight at 8 weeks, when the animals were sacrificed. * and ** are 0.05. C, Caski tumors prepared from CB injected NOD/SCID mice were analyzed by flow cytometry with anti-human CD19 and CD3, or CD4 and CD8 antibodies. D, immunohistochemical analysis of tumors in mice was detected the presence of high level of tumor infiltrated CD8+ T cells and CD4+ T cells, derived from CB mononuclear cells on 0 (left) and 14 (right) days after Caski injections. Hoechst dye was used for counterstaining of cell nuclei.