Figure 5
The FOXO3-p53(mut) complex promotes p27kip1 expression. (A) The protein-DNA complex was immunoprecipitated with antibody against p53 and DNA was amplified by conventional PCR with primers from the p27kip1 promoter region (ChIP assay) with input representing PCR amplification from lysate before immunoprecipitation with the same primers. The FOXO3-p53(mut) complex is bound to the p27kip1 promoter. (B) HT-29 cells transfected with p53 siRNA or scramble siRNA were treated with genistein and examined for p27kip1 by immunoblot. Silencing of p53 attenuates genistein-induced p27kip1 expression. These experiments were repeated two independent times.