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Table 2 Quantitative and qualitative characteristics of RNA used for RT-qPCR analysis: median [min - max]

From: Importance of pre-analytical steps for transcriptome and RT-qPCR analyses in the context of the phase II randomised multicentre trial REMAGUS02 of neoadjuvant chemotherapy in breast cancer patients

Characteristics Centre All centres
  1 2 3 4  
Tumour
cells (%)
60
[30 - 95]
60
[30 - 90]
50
[30 - 90]
70
[30 - 90]
60
[30 - 95]
RIN 8.40
[6.10 - 9.90]
8.90
[6.70 - 10.0]
8.00
[6.00 - 9.30]
8.30
[6.40 - 9.30]
8.40
[6.00 - 10.00]
260/280 ratio 2.08
[1.99 - 2.13]
2.05
[1.72 - 2.17]
2.08
[2.02 - 2.15]
2.09
[1.94 - 2.14]
2.08
[1.72 - 2.17]
28 s/18 s ratio 1.60
[0.70 - 2.10]
1.70
[1.10 - 2..40]
1.35
[1.00 - 1.80]
1.30
[0.90 - 2.00]
1.60
[0.70 - 2.40]
RNA (μg) 16.77
[2.49 - 89.58]
11.02
[3.15 - 31.95]
13.92
[5.00 - 26.93]
6.90
[3.60 - 20.34]
12.52
[2.49 - 89.58]