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Table 2 Quantitative and qualitative characteristics of RNA used for RT-qPCR analysis: median [min - max]

From: Importance of pre-analytical steps for transcriptome and RT-qPCR analyses in the context of the phase II randomised multicentre trial REMAGUS02 of neoadjuvant chemotherapy in breast cancer patients

Characteristics

Centre

All centres

 

1

2

3

4

 

Tumour

cells (%)

60

[30 - 95]

60

[30 - 90]

50

[30 - 90]

70

[30 - 90]

60

[30 - 95]

RIN

8.40

[6.10 - 9.90]

8.90

[6.70 - 10.0]

8.00

[6.00 - 9.30]

8.30

[6.40 - 9.30]

8.40

[6.00 - 10.00]

260/280 ratio

2.08

[1.99 - 2.13]

2.05

[1.72 - 2.17]

2.08

[2.02 - 2.15]

2.09

[1.94 - 2.14]

2.08

[1.72 - 2.17]

28 s/18 s ratio

1.60

[0.70 - 2.10]

1.70

[1.10 - 2..40]

1.35

[1.00 - 1.80]

1.30

[0.90 - 2.00]

1.60

[0.70 - 2.40]

RNA (μg)

16.77

[2.49 - 89.58]

11.02

[3.15 - 31.95]

13.92

[5.00 - 26.93]

6.90

[3.60 - 20.34]

12.52

[2.49 - 89.58]

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BMC Cancer

ISSN: 1471-2407

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