Figure 1

Western blot analysis of GIST882 cell lysate compared to a patient serum using the biotinylated antibody L1-UJ127. Three major bands corresponding to L1 are detectable in the cell lysate. The 220 kDa form represents the full length L1-Protein, while L1-180 kDa and L1-80 kDa are proteolytically cleaved. L1-180 kDa represents the shedded extracellular domain while L1-80 kDa is a c-terminal fragment integral to the membrane. In serum, soluble forms of L1 with the molecular weights of approx. 200 kDa and 180 kDa are detectable. The L1-80 kDa fragment is not present in sera.