Figure 5

Effects of TIG1A and GRK5 siRNAs on TIG1A-mediated suppression of HCT116 cell growth. (A) TIG1A cells were transfected with the indicated siRNA after which they were immediately incubated with MFP (5 nM) for 24 h. Expression of TIG1A, GRK5, and actin was examined by Western blot analysis using anti-myc, anti-GRK5, and anti-actin antibodies, respectively. (B) Inducible TIG1A stable cells were plated in triplicate overnight, and then transfected with the indicated siRNA, and incubated in the presence or absence of 5 nM MFP for 48 h. Cell proliferation was measured using the WST-1 method. Relative levels of cell growth were normalised to that of control siRNA transfected cells without MFP treatment. *, P < 0.05 and ***, P < 0.001.