Figure 5

Marker expression in different zones around the vessels. Zone analysis in a biopsy with a large hypoxic fraction (upper panels) and a tumor with no pimonidazole staining (lower panels). Pimonidazole staining increases steadily at larger distances from the vessels (a). The increase of GLUT, MCT4 and LDH-5 expression is less steep with a plateau reached between 150-200 μm (b). MCT1 expression increases until 150-200 μm, a slight decrease is noted at larger distances (a). In the tumour with no pimonidazole staining, a decrease in MCT1 expression (c) and no increase of GLUT, MCT4 and LDH-5 expression (d) is observed at larger distances from the vessels.