Figure 4

Effects of CD248 on Notch3 signaling. Baseline transcript levels of Notch3 (A), Jagged1 (B), Hes (C) and Hey1(D), are increased in CD248^CyD/CyD fibroblasts as compared with CD248^WT/WT fibroblasts(n = 4, p = 0.0139 for Notch3; p = 0.0041 for Jagged1; p = 0.00067 for Hes; p = 0.0051 for Hey1). Fibroblasts were also co-cultured with human EaHy926 endothelial cells for 24 hours (A-D), causing further upregulation of the transcripts for both genotypes. E. Transcript levels of CD248 in CD248^WT/WT and CD248^CyD/CyD fibroblasts are significantly reduced to similar levels after co-culture with EaHy926 endothelial cells (n = 4).