Signaling pathways and gene expressions regulated by IMOS. (A) pERK, tERK, and pJNK were significantly down-regulated by IMOS in both HepG2 and Hep3B cells, whereas c-MET was markedly down-regulated only in HepG2 cells. No obvious changes were found in protein levels of pAkt, tAkt, and tJNK. (B) Ten genes were significantly regulated by IMOS in HCCLM3-RFP xenografts. Among them, Bcl-2, BIRC5/survivin, PCNA, CDK1/CDK2, and PRC1 were down-regulated, and BAI-1, TP73, and MDM2 up-regulated by more than twice. (C,D) Except for RPRM/Reprimo and IFN-β, similar expression profiles were confirmed in IMOS-treated HepG2 and Hep3B cells.