Effects of CCM and DHA on p53 and PPARγ. SK-BR-3 (A) and MCF-7 (B) cells were treated with 30 μM DHA, CCM, or the CCM+DHA combination (12 μM CCM and 18 μM DHA) for 24 hours in 6 well plates. (C) SK-BR-3 cells were treated with escalating doses of CCM or DHA individually. Cells were lysed and analyzed by SDS-PAGE. Blots were probed for phosphorylated p53, overall p53, p21, PPARγ, and GAPDH as indicated. Data are representative of three separate duplicate experiments.