Figure 4

CCM+DHA inhibit proliferation through a caspase-mediated process. SK-BR-3 cells were pretreated for one hour with 20 μM Z-VAD-FMK, a pan-caspase inhibitor, prior to the addition of escalating doses of CCM+DHA (2:3 ratios). After 24 hour incubation, proliferation was analyzed with WST-1 reagent according to manufacturer protocol. Results are representative of three separate triplicate experiments. *P < 0.05 for Student's t-tests comparing the treatments with or without the pan-caspase inhibitor.