Curcumin affects microtubule dynamics and mitotic spindle assembly. A. Immunofluorescence for β-tubulin in DAOY cells treated with DMSO (0 μM) or curcumin (10, 20 μM) for 6 hours. Bar, 20 μm. B. Analysis of mitotic spindle microtubules in DAOY cells. Mitotic cells were released from the G2/M arrest in the presence or absence of curcumin. The cells were fixed after 60 min of release and stained for β-tubulin (red) and DNA (blue). The arrow indicates segregation of chromosomes along the mitotic spindle, while the arrowhead shows abnormal spindle formation and missegregation of chromosomes. Bar, 20 μm. C. Quantitative analysis of cells with abnormal mitotic spindles after 60 min of incubation with either vehicle or 20 μM curcumin. Error bars indicate standard deviations of three independent experiments.