Curcumin induces apoptosis in medulloblastoma cells. A. Phase contrast images of DAOY cells incubated with indicated concentrations of curcumin for 16 hours. Bar, 100 μm. B. LDH release as measure of the cytotoxic effect of curcumin. C. Immunoblot of cleaved caspase-3 and PARP in curcumin-treated DAOY cells. α-tubulin immunoblot confirms that equal amounts of protein were used in the analysis. D. Immunofluorescence of control and curcumin-treated DAOY cells (16 hours) for cleaved capase-3 (green) and F-actin (red). Bar, 20 μm.