CpG methylation modifications correlate with LXN gene silencing in human immortalized and cancerous cells. (A) Schematic diagram of the LXN gene structure derived from the GenBank database. The 26 CpG dinucleotides in exon 1 and the region upstream of exon 1 selected for bisulfite sequencing are indicated in the CpG map. (B) Relative latexin expression in cell lines was evaluated using Western blot normalized to Actin protein level (shown in Figures 1B and 1C). (C) Quantitative measurement of methylation of 26 CpG dinucleotides. Filled and open square boxes indicate methylated and unmethylated CpG sites, respectively. Each row of boxes represents a single clone. Numerical values on the right show the average percentage of methylated CpG sites of each indicated cell line.