The effect of downregulated latexin expression on tumor cell growth and tumorigenicity of BGC823 cells. (A) Gastric cancer cells BGC823 were transfected with an antisense LXN gene expressing vector (pLXSN-antisense-LXN) and two G418-resistant colonies (C3 and C7) with stably decreased latexin expression were selected and tested by Western blot using 1G11 antibody (1:1000 dilution). BGC823 cells transfected with empty pLXSN vector were used as control. (B) C3, C7, and control BGC823 cells were used for colony formation assay. Assays were performed in triplicate and the average numbers of clones were calculated and shown in the graphs. (C) C3, C7, and control BGC823 cells were injected subcutaneously into BALB/c nude mice (8 mice/group). The left graph shows tumor volume as measured on the indicated days. Tumors were excised 21 days after injection (middle panel) and the average weight of tumors of each group was shown as columns (right panel), with bars representing Standard Deviation.