The effect of ectopic latexin expression on tumor cell growth and tumorigenicity of MGC803 cells. (A) Latexin expression in gastric cancer cells MGC803 transfected with empty vector (pcDNA4/TO) and MGC803 cells stably overexpressing latexin (C39-8 and C46) was tested by Western blot using 1G11 antibody (1:1000 dilution). (B) C39-8, C46, and control MGC803 cells were used for anchorage-dependent colony formation assay. The photographs show colonies formed by each stable transfectant 2 weeks after plating. Assays were performed in triplicate and the average numbers of clones from one whole well are shown in the graph. (C) C39-8, C46, and control cells were injected subcutaneously into BALB/c nude mice (8 mice/group). The graph (left panel) represents tumor volume as measured on the indicated days. Tumors were excised 21 days after injection (middle panel) and the average weight of tumors of each group was shown as columns (right panel), with bars representing Standard Deviation.