Figure 2

The putative KLK15 promoter region: (A) The 11 kb region upstream of exon 1 on chromosome 19 (56020307 to 56037591 bp, NCBI build 36.3) was downloaded for in silico analysis (A) schematic of the 5' region of the KLK15 gene with significant motifs noted: 2 putative Androgen Response Elements (AREs) were found by both JASPAR and Cister programs (red arrows); 4 putative Estrogen Response Elements (EREs) were found by both ERE finder and Cister programs (blue arrows); 2 putative cis-element clusters which indicate putative promoter regions found by Cister (green arrows); 2 putative promoter regions that overlap with CpG islands found by WWWPromoter Scan, Promoter Inspector and CpG Island Finder (yellow arrows) (B) AREs (boxes), EREs (triangle) and Nuclear Hormone Receptor Binding Sites (NHRBs) (circles) as predicted by individual softwares. The ARE "cluster" consists of 16 AREs. The colored bars (blue, red, green, orange, yellow) in the sequence indicate the 5 regions that were chosen for sequencing genomic DNA. The single nucleotide polymorphism (SNP)-modeling results are shown by color coding the functional SNPs validated in bold. Orange and red text coloring indicate a gain or loss of ERE motifs respectively, while green and blue indicate ARE gain or loss. The two brown SNPs indicate a loss of one ERE and a loss of two AREs. An additional four validated SNPs viz rs2659052, rs11880663, rs2659051, rs2659055 have been added to NCBI databases (not modeled here) from the time of our analysis.