Figure 15
Effect of huMETCAM/MUC18 expression on a disorganized growth of MDA-MB-231 and MDA-MB-468 cell lines in 3D basement membrane culture assay. The embedded 3D basement membrane culture assay was carried out as described in "Materials and Methods". Panels A and D, in which no antibody was added, show a disorganized growth of both MDA-MB-231 and MDA-MB-468 cells in 3D culture assay, respectively. Panels B and E, in which the control isotype antibody (Chicken IgY) was added, show results similar to Panels A and D. Panels C and F show that the disorganized growth of both MDA-MB-231 and MDA-MB-468 cells in 3D culture assay was destroyed in the presence of 15 μg/ml of anti-huMETCAM/MUC18 antibody, respectively.