ABI3 ectopic expression reduced cell proliferation, cell viability and arrested cells in G0/G1 phase. (A) Growth of cells is suppressed following ABI3 expression, mainly in WRO cell line. (B) The percentage of apoptotic cells was not significantly altered in cell lines expressing ABI3, compared to control cells. (C) ABI3 expression reduced cell viability in ARO cells, mainly at day 5 where a more evident effect was observed in the proliferation assay. (D) ABI3 expression increased the percentage of cells in G0-G1 at the expense of G2-M phase. Black bars correspond to control clones (n = 2) and white bars correspond to ABI3 expressing clones (n = 2). *p < 0.05 and *** p < 0.001.