Figure 6
From: Interaction and uptake of exosomes by ovarian cancer cells
Effects of neuraminidase and sugars on SKOV3 exosomes uptake. (A) Exos-CFSE (20 ÎŒg protein) were incubated with 15 mU V. cholerae neuraminidase or corresponding buffer for 2 h before Exos-CFSE uptake. (B) Effect of 150 mM D-glucose (Glc), α-L-fucose (Fuc), α-D-mannose (Man), ÎČ-lactose (Lac), D-galactose (Gal) and D-N-acetylglucosamine (GlcNAc) (30 min pre-incubation) on Exos-CFSE uptake (4 h) monitored by flow cytometry analysis. Uptake efficiency was calculated relatively to the uptake without treatment, considered as 100%. Results are displayed in relative percentages ± S.D. The results shown are representative of three independent experiments performed in duplicate.