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Figure 2 | BMC Cancer

Figure 2

From: The investigation of Mitogen-Activated Protein kinase Phosphatase-1 as a potential pharmacological target in non-small cell lung carcinomas, assisted by non-invasive molecular imaging

Figure 2

MKP-1 regulates expressions of MMP-2 and CXCR4 in H441GL cell. H441GL cells were treated with a p38MAPK (SB203580), an ERK1/2 inhibitor (PD98059), or a JNK (SP600125) (all at 30 μM) (A) Cell lysates were immunoblotted with antibodies specific for MMP-2, CXCR4, or α-tubulin Inhibitors against MAKPs all reduced both CXCR4 and MMP-2 expression levels except inhibitor against JNK did not significantly reduced MMP-2 expression Corresponding MMP-2 activity was measured using zymography (B). (C) MMP-2 and CXCR4 expression were examined in MKP-1 over-expressing H441GL cells Decreased expression levels of both molecules were detected in wildtype MKP-1 over-expressing H441GL cells (H441GL/MKP-1) when compared to those of in vector control (H441GL/pcDNA31) and dominant negative form of MKP-1 (H441GL/MKP-1CS) Corresponding MMP-2 enzymatic activity was demonstrated by zymography (D).

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BMC Cancer

ISSN: 1471-2407

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