Figure 2

Subcellular localization of hepaCAM and its extracellular domain truncation mutant in T24 cells. In well-spread cells, hepaCAM and its extracellular domain truncation mutant were localized on the perinuclear membrane and at the tip of cell surface protrusions. [B, C (white arrows indicate the localization of wild-type hepaCAM and its mutant), A is the control group]. In confluent cells, hepaCAM predominantly accumulated at the sites of cell-cell contacts on the cell membrane (E). However, in confluent cells, hepaCAM-mt1 did not accumulate at the sites of cell-cell contact, but instead localized on the cell membrane non-specifically (F, white arrows indicate localization of hepaCAM-mt1). D is the control group. 1000× magnification.