Figure 3

Dose-dependent responses of TCPTP expressing and null MEFs to TCPTP agonist. Four TC45 activating compounds: spermidine, ruthenium red, mitoxanthrone; and MDL-26,630-trihydrocloride were tested for their effect on cell proliferation in TCPTP wild-type (O) and knockout (O) mouse embryonic fibroblasts (MEFs). Wildtype and knockout MEFs were incubated in serum-containing medium in the presence of the HTS-identified compounds, as indicated for 72 h. Cells were detected with Cell Titer Blue reagent, and the non-linear regression analysis was done using GraphPad Prism (mean ± SD of 3 experiments with similar results).