High-throughput screening. A. Overview of the high-throughput screening method used. Five small molecule libraries, the Spectrum Collection, LOPAC1280, ChemDiv, ChemBridge and Tripos were screened for TCPTP activators. TCPTP was added to 384-well plates and background fluorescence was measured to control for compound interference due to autofluorescence. DiFMUP phosphatase substrate was added, and the reaction was stopped with urea prior to measuring fluorescence with EnVision. Integrin α1-cyt peptide was added as a positive control to each assay plate. B. Shown are the combined results of all library screens. The data points circled in red represent the 6 confirmed TCPTP activator hits. Data was normalised using the B-score method. C. The chemical structures of the 6 confirmed TCPTP activator hits.