Activation of the IGF-1 pathway in PC3-AKR1C3 transfectants. (A) Ingenuity analysis identified that activation of the IGF-1 signaling pathway is statistically significant in PC3-AKR1C3 transfectants. Up-regulated genes, or focus genes, are marked by in filled gray; and uncolored genes indicate they are expressed in the microarray datasets under any conditions and may participate in signal transduction. Branches of the IGF-1 pathway that did not have at least one focus gene were not included in the diagram. (B) Western blot analysis of total and phosphorylated IGF-1R β and Akt in PC3-AKR1C3 stable transfectants. The analysis was performed 3 times; and all experiments showed consistent elevated phosphorylation of IGF-1R β (Tyr 1131) and Akt (Ser 473) in PC3-AKR1C3 transfectants. Image analysis confirmed that levels of phosphorylated IGF-1R β and Akt are statistically higher in PC3-AKR1C3 transfectants as compared to PC3-mock transfectants.