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Table 1 Expression of both angiotensin II AT1 and AT2 receptors was detected in wild-type mouse skin fibroblasts (MSF) but not in PAN02 cells

From: Angiotensin II type 2 receptor signaling significantly attenuates growth of murine pancreatic carcinoma grafts in syngeneic mice

Genes AT1 AT2 18S
Serum + - + - + -
MSF 22.8 23.5 28.1 20.6 11.9 12.8
PAN02 29.6 29.7 29.4 29.7 10.0 11.3
H20 30.6 30.0 29.8
  1. Ang II receptor expression in wild-type MSF and PAN02 cells was determined by real-time PCR and expressed as threshold cycle (Ct) value. The 18S ribosomal RNA was used as an internal standard. The Ct value indicates the fractional cycle number at which the amount of amplified target reaches a fixed threshold. Cells were cultured in either serum-containing or serum-free medium for two days, total RNA was extracted, and the receptor expression was determined. The values represent the average of triplicate determinations. Serum-free culture is known to increase AT2 expression in cultured cells [34].