Angiotensin II type 2 receptor signaling significantly attenuates growth of murine pancreatic carcinoma grafts in syngeneic mice

Table 1 Expression of both angiotensin II AT₁ and AT₂ receptors was detected in wild-type mouse skin fibroblasts (MSF) but not in PAN02 cells

Genes	AT₁		AT₂		18S
Serum	+	-	+	-	+	-
MSF	22.8	23.5	28.1	20.6	11.9	12.8
PAN02	29.6	29.7	29.4	29.7	10.0	11.3
H₂0	30.6		30.0		29.8

Ang II receptor expression in wild-type MSF and PAN02 cells was determined by real-time PCR and expressed as threshold cycle (Ct) value. The 18S ribosomal RNA was used as an internal standard. The Ct value indicates the fractional cycle number at which the amount of amplified target reaches a fixed threshold. Cells were cultured in either serum-containing or serum-free medium for two days, total RNA was extracted, and the receptor expression was determined. The values represent the average of triplicate determinations. Serum-free culture is known to increase AT₂ expression in cultured cells [34].

ISSN: 1471-2407