The effect of Ang II and an AT
receptor antagonist on VEGF protein expression in mouse fibroblasts was determined by Western blot analysis. Primary cultured mouse skin fibroblasts were treated with Ang II (10 nM) in the presence or absence of the AT1- and AT2-specific antagonists Losartan (1 μM) and PD123,319 (10 μM), respectively, as indicated in the figure. VEGF protein was determined 48 h after Ang II treatment by Western blot analysis as described in the Methods. The average (means ± SEM) of two separate experiments is displayed in the histogram. Ang II attenuated VEGF expression (P = 0.1), but this attenuation was completely blocked by the AT2 antagonist PD123,319. However, PD123,319 treatment significantly increased VEGF production as compared to untreated control (*, P ≤ 0.05).