Figure 5
The effect of Ang II and an AT 2 receptor antagonist on cell proliferation, as determined by MTT assay, of PAN02 cells (black solid bars) co-cultured with MSFs prepared from either wild type (WT) mice (untransfected WT fibroblasts, WT, open bars; and AT 2 over-expressing WT fibroblasts, AT2 WT, half-shaded bars) or AT 2 -KO mice (untransfected AT 2 -KO fibroblasts, AT 2 KO, dark grey bars; and AT 2 over-expressing AT 2 -KO fibroblasts AT2 AT 2 KO, hashed bars). Untransfected or AT2 transfected primary cultured mouse skin fibroblasts were cultured one day prior to the initiation of PAN02 co-culture. The ratio of fibroblasts to PAN02 cells was 1:4. The cells were treated with Ang II (10 nM) in the presence or absence of the AT2-specific antagonist PD123,319 (10 μM) as indicated in the figure. Cell proliferation was determined 72 h after Ang II treatment by MTT assay as described in the Methods. The average (means ± SEM) of three separate experiments is displayed in the histogram. Cell proliferation of PAN02 co-cultured with AT2 over-expressing fibroblasts was significantly lower than that with untransfected fibroblasts regardless of the cell source (*, P ≤ 0.05), whereas PAN02 proliferation was significantly increased when cells were treated with the AT2 antagonist PD123,319 (** P ≤ 0.01).