Figure 4

Translocation of GFP-LC3 after treatment with C-DIMs. Translocation of transiently transfected GFP-LC3 plasmid. GFP-LC3 was transiently transfected into MDA-MB-231 [A] and MDA-MB-453 [B] and cells were treated with DMSO or 10 μM C-DIMs as outlined in the Materials and Methods. Confocal microscopy images of live cells were captured after treatment with C-DIMs for 24 h and differential interference contrast (DIC) and DIC/GFP overlay images of the same microscopic field are illustrated. At least 3 areas were scanned per well and the images depicted are representative of treatment replicates. Width of each field is 100 μm.