|
Samples
|
OCT4/POU5F1
|
NANOG
|
HTERT
|
CD133/PROM1
|
|---|
|
TG1
|
5937 a
|
1240
|
131
|
5
|
|
TG6
|
11857
|
1959
|
165
|
12
|
|
TG10
|
1686
|
169
|
517
|
3
|
|
TG16
|
871
|
611
|
73
|
14
|
-
OCT4/POU5F1, NANOG, HTERT and CD133/PROM1 genes expressions were evaluated using real-time quantitative PCR in 4 MGNT-derived cells forming spheres. Tests were done with cultures at passage 30 ± 5. Data are from one experiment out of three giving similar results. a N-fold differences in target gene expression relative to the smallest amount of target gene mRNA detectable and quantifiable by real-time quantitative RT-PCR assays (target gene Ct value = 35; Ntarget value = 1).
