Samples |
OCT4/POU5F1
|
NANOG
|
HTERT
|
CD133/PROM1
|
---|
TG1
| 5937 a
| 1240 | 131 | 5 |
TG6
| 11857 | 1959 | 165 | 12 |
TG10
| 1686 | 169 | 517 | 3 |
TG16
| 871 | 611 | 73 | 14 |
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OCT4/POU5F1, NANOG, HTERT and CD133/PROM1 genes expressions were evaluated using real-time quantitative PCR in 4 MGNT-derived cells forming spheres. Tests were done with cultures at passage 30 ± 5. Data are from one experiment out of three giving similar results. a N-fold differences in target gene expression relative to the smallest amount of target gene mRNA detectable and quantifiable by real-time quantitative RT-PCR assays (target gene Ct value = 35; Ntarget value = 1).