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Table 4 Expression of stemness markers by MGNT-derived cells forming spheres.

From: CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors

Samples OCT4/POU5F1 NANOG HTERT CD133/PROM1
TG1 5937 a 1240 131 5
TG6 11857 1959 165 12
TG10 1686 169 517 3
TG16 871 611 73 14
  1. OCT4/POU5F1, NANOG, HTERT and CD133/PROM1 genes expressions were evaluated using real-time quantitative PCR in 4 MGNT-derived cells forming spheres. Tests were done with cultures at passage 30 ± 5. Data are from one experiment out of three giving similar results. a N-fold differences in target gene expression relative to the smallest amount of target gene mRNA detectable and quantifiable by real-time quantitative RT-PCR assays (target gene Ct value = 35; Ntarget value = 1).
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